BiochemistrybioRxivPreprint
Optimized Mn2+-Phos-tag Gels Reveal Sarcomeric Protein Dephosphorylation upon Myofibril Preparation
Sunayana Begum Syed, Axel J. Fenwick, Skylar M.L. Bodt…·D. Brian Foster
This paper optimizes Mn2+-Phos-tag SDS-PAGE for cardiac myofilament proteins, showing that myofibril preparation causes significant dephosphorylation of MLC2 and cTnI.
DOI 10.64898/2026.08.21.746362