Author

Ralph Wieneke

Goethe University FrankfurtORCID 0000-0002-0767-4679Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Research Focus:cell type annotation· 1 Assay:fluorescence / live-cell microscopy· 1 Organism:human· 1 Research Focus:imputation· 1 Research Focus:protein design· 1 Assay:proteomics· 1

Frequent Co-Authors

Robert TampéiD· 2 shared Alina Klein· 1 shared Anika Raulf· 1 shared Federica GasparriiD· 1 shared Felicitas Tissen· 1 shared Han Chow ChuaiD· 1 shared Hendrik Johannes HarmsiD· 1 shared Iacopo GalleanoiD· 1 shared

Activity by Year

2017-2020 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 27 mutual co-author links

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2 shared papersSynthetic Biology

Robert TampéORCID

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2 papers

Recent·Most cited

Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing

Keith K. Khoo, Iacopo Galleano, Federica Gasparri, Ralph Wieneke, Hendrik Johannes Harms, Mette Homann Poulsen, Han Chow Chua, Matthias Wulf, Robert Tampé, Stephan Alexander Pless

This paper introduces a method to insert synthetic peptides into proteins in eukaryotic cells to enable chemical modifications and non-canonical amino acids, validated using GFP, NaV1.5, and P2X2 receptors.

Nature Communications · May 8, 2020Peer-reviewedSynthetic Biology

Multiplexed Live-Cell Visualization Of Endogenous Proteins With Nanometer Precision By Fluorobodies

Alina Klein, Susanne Hank, Anika Raulf, Felicitas Tissen, Mike Heilemann, Ralph Wieneke, Robert Tampé

This paper introduces fluorobodies, engineered nanobodies with bright fluorophores, enabling high-precision live-cell imaging of endogenous proteins with low background and toxicity.

bioRxiv · Jun 2, 2017 · PreprintCell Biology