Synthetic BiologyNature CommunicationsPeer-reviewed
Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing
Keith K. Khoo, Iacopo Galleano, Federica Gasparri…·Stephan Alexander Pless
This paper introduces a method to insert synthetic peptides into proteins in eukaryotic cells to enable chemical modifications and non-canonical amino acids, validated using GFP, NaV1.5, and P2X2 receptors.
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