Robert Tampé

Kinetic asymmetry drives directionality in an ATP-binding cassette transporter

The paper identifies ionic locks in ABC transporters that govern directionality through kinetic asymmetry in ATP binding and hydrolysis.

Reconstitution of glycan-driven MHC I recycling reveals calreticulin as mediator between TAPBPR and tapasin

The paper reveals calreticulin mediates glycan-dependent transfer of MHC I from TAPBPR to tapasin, essential for MHC I recycling and quality control.

ATP-driven conformational dynamics reveal hidden intermediates in a heterodimeric ABC transporter

This study uses single-molecule FRET to analyze ATP-driven conformational dynamics of TmrAB, revealing hidden intermediates and long ATP-bound states.

Braess' Paradox in Enzyme Kinetics: Asymmetry from Population Balance without Direct Cooperativity

The paper uses Bayesian Markov models to explain asymmetric ATP hydrolysis kinetics in ABCE1 without requiring direct cooperativity between nucleotide-binding sites.

Single-molecule dynamics reveal ATP binding alone powers substrate translocation by an ABC transporter

The paper uses single-molecule FRET to show ATP binding alone drives peptide translocation by TmrAB without Mg2+.

US6 hijacks the peptide-loading complex by trapping transporter-chaperone dynamics

This paper reveals how the HCMV protein US6 inhibits the peptide-loading complex by altering TAP dynamics, providing insights into viral immune evasion mechanisms.

Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing

This paper introduces a method to insert synthetic peptides into proteins in eukaryotic cells to enable chemical modifications and non-canonical amino acids, validated using GFP, NaV1.5, and P2X2 receptors.

Cytosolic delivery of large supramolecular proteincomplexes arranged on DNA nanopegboards

This paper describes a method for delivering large protein complexes into cells using DNA nanostructures and microfluidic squeezing, demonstrating delivery into HeLa cells and potential applications in immunotherapy.

Multiplexed Live-Cell Visualization Of Endogenous Proteins With Nanometer Precision By Fluorobodies

This paper introduces fluorobodies, engineered nanobodies with bright fluorophores, enabling high-precision live-cell imaging of endogenous proteins with low background and toxicity.