Long Read Sequencing reveals transgene concatemerization and backbone integration following AAV-driven electroporation of CRISPR RNP complexes in mouse zygotes
Muhammad Waqas Luqman, Piroon Jenjaroenpun, Jessica Spathos, Nikhil Shingte, Mitchell J Cummins, Pattaraporn Nimsamer, Lars Matthias Ittner, Thidathip Wongsurawat, Fabien Delerue
The paper uses long-read sequencing to analyze unintended on-target mutations in mouse zygotes edited with AAV-delivered CRISPR RNP complexes.
Frontiers in Genome Editing · Jun 4, 2025Peer-reviewedBioengineering