Tomoki Ohkubo

This paper demonstrates a hybrid in silico/in-cell controller that reduces toxicity from excessive enzyme overexpression by adapting to process-model mismatch in engineered E. coli.

This paper introduces a hybrid control system for microbial bioprocesses that addresses process-model mismatch by combining in silico and in-cell controllers.

The paper develops a microfluidic system to control extracellular signal molecule concentrations and measures intracellular Ca2+ responses in CHO-K1 cells.