Shivang Hina-Nilesh Joshi

A genome-integrated Cas12m in E. coli shows no measurable growth cost, reverses repression faster than dCas9, and supports NOT gates, a two-layer buffer and a two-pathway pigment circuit.

The paper develops a workflow combining robotics, nanopore sequencing, and computational tools to characterize recombinase-based genetic circuits efficiently and at high resolution.

The paper introduces SHARK, a series of Pir E. coli strains designed for efficient cloning of R6K plasmids, showing they are >100-fold more efficient than a commercial strain for complex cloning.