David Ulaeto

A genome-integrated Cas12m in E. coli shows no measurable growth cost, reverses repression faster than dCas9, and supports NOT gates, a two-layer buffer and a two-pathway pigment circuit.

The paper introduces SHARK, a series of Pir E. coli strains designed for efficient cloning of R6K plasmids, showing they are >100-fold more efficient than a commercial strain for complex cloning.

This study compares a 2022 MPXV lineage B.1 virus with its clade IIb ancestor A.1, showing attenuated phenotypes including reduced virulence and immune evasion.