Site-specific metabolic labeling reveals proteome-wide phospho-dynamics
Mihai Alevra, Miguel Correa Marrero, Verena Kluever, Sunit Mandad, Nisha Hemandhar-Kumar, Kuan‐Ting Pan, Julian van Gerwen, Selda Kabatas, Hanna Wildhagen, Till Ischebeck, Yansheng Liu, Silvio O. Rizzoli, Henning Urlaub, Pedro Beltrão, Eugenio F. Fornasiero
This study introduces PulsPhos, a method to quantify site-specific phosphorylation lifetimes in living cells and applies it to pharmacological perturbations.
bioRxiv · Jul 23, 2024 · PreprintMolecular Biology