Eugenio F. Fornasiero

The paper introduces MEMBRYO, a method for continuous SILAC proteomics in live embryos, revealing post-transcriptional regulation during neurogenesis.

This study shows that circadian disruption in rats alters the rhythmic expression of clock and memory genes in the hippocampus without changing overall expression levels.

This paper introduces sdAb(mNG), a high-affinity nanobody that enables versatile applications for mNG-tagged proteins, including imaging, immunoprecipitation, and in vivo manipulation.

This study shows that nanotopography regulates pluripotency in mESCs by altering chromatin state through the mechanosensitive demethylase KDM3A.

This study identifies molecular signatures of aging in the mouse brain through proteo-transcriptomic analysis, revealing changes in protein aggregation, mRNA relocalization, and translation efficiency linked to neurodegeneration.

The paper introduces IVEA, a deep-learning platform for automated exocytosis analysis in live-cell imaging, which is faster and more reliable than manual methods.

The paper introduces NbLumSyt1 nanobinders for analyzing synaptic vesicle dynamics in rodent and human models with improved precision and versatility.

This study investigates changes in the synaptic proteome and transcriptome during aging, revealing decoupling between transcripts and proteins and altered ribosomal gene distribution.

The paper discovers that levetiracetam prevents Aβ42 production by modulating synaptic vesicle cycling, altering APP localization, and favoring non-amyloidogenic processing in Alzheimer's disease models.

The paper presents Turnover-PPT, a resource mapping protein and phosphoprotein turnover across mouse tissues and brain regions, revealing tissue-specific protein lifetimes and phosphorylation's impact on stability.

This study introduces PulsPhos, a method to quantify site-specific phosphorylation lifetimes in living cells and applies it to pharmacological perturbations.

This paper introduces SUM-PAINT, a high-throughput imaging method for single-protein spatial resolution, and discovers a new synapse type through extensive multiprotein analysis.

This paper introduces a nanobody that enables non-invasive live synaptic imaging and improves super-resolution microscopy with minimal physiological impact.

The paper develops DELTA, a method to measure brain-wide synaptic protein turnover at single-synapse resolution, revealing localized plasticity in hippocampal CA1 during learning.

This study shows that IGF1R regulates firing rate homeostasis by modulating mitochondrial calcium uniporter function in neurons.

The paper develops a fluorescent reporter (FluoReSyn) using a nanobody to detect human α-synuclein in cells and tissues, showing its presence in cerebrospinal fluid.

The paper shows that aged synaptic vesicles are inactivated by SNAP25 contamination, which inhibits exocytosis and promotes co-localization with recycling endosomes.