Silvio O. Rizzoli

The paper shows that alpha-synuclein phase separation can form Lewy body-like structures without pre-existing seeds, suggesting a new mechanism for neurodegeneration.

This study investigates how synapsin-1 and actin form ordered nanoscale assemblies through phase separation, showing that actin scaffolds retain structural memory for condensate reformation.

The paper introduces Mega-expansion microscopy, a fluorescence imaging technique that enables high-resolution structural visualization of whole cells using conventional microscopes.

A four-network sodium acrylate/dimethylacrylamide hydrogel, cast ionic-within-ionic without neutral re-embedding, reaches about 1000-fold linear expansion and resolves individual peptide residues on standard confocal microscopes.

This paper introduces a single-step expansion microscopy method enabling SMLM with spontaneously blinking dyes in expanded samples, achieving nanometer-resolution imaging of cellular structures.

The paper introduces a data-driven framework to predict collective synaptic plasticity outcomes by considering protein allocation and spatial competition among synapses.

The paper shows synaptic vesicles from mouse brains can sequester soluble tubulin in vitro, forming tubulin-rich regions.

SynapseNet automatically segments synapses in electron micrographs using deep learning, enabling efficient analysis of synaptic structures.

The paper investigates how synapsin-SV condensates organize presynaptic actin through LLPS, revealing their role in SV clustering and actin polymerization in lamprey and mammalian synapses.

This study overexpresses human alpha-synuclein in rodent glutamatergic neurons and finds no significant impairment in synaptic function.

The paper develops an optimized Ex-dSTORM method to resolve endogenous protein assemblies with nanometer precision in cells.

This study uses in silico FRAP experiments to analyze how synaptic geometry and protein-vesicle binding affect protein mobility, identifying two key mechanisms influencing recovery times.

The paper models the synaptic vesicle cycle with high spatial and molecular detail, showing dynamic recycling and regulation by synapsin-1 and tomosyn-1 during sustained activity.

This paper introduces a knock-in mouse line with endogenous Munc13-1-SNAP for monitoring presynaptic nanoarchitecture using SNAP-tag labeling and live-cell imaging.

The paper introduces NbLumSyt1 nanobinders for analyzing synaptic vesicle dynamics in rodent and human models with improved precision and versatility.

The paper models synaptic vesicle recycling dynamics and shows that multi-timescale recovery can be described by a power-law adaptation model, which enables efficient neuronal communication through temporal whitening of spike trains.

The paper develops a correlative cryo-light/electron microscopy workflow to target and image synapses with cryo-ET, revealing excitatory synapses.

The paper investigates whether actin dynamics and spine geometry can serve as a synaptic tag for long-term potentiation (LTP) by combining mathematical modeling and FRAP experiments.

This study shows that neddylation is essential for the development and function of glutamatergic neurons, with Nedd8 deletion causing defects in dendrite complexity and synaptic transmission.

The paper introduces MJF-14 PLA, a proximity ligation assay for detecting non-inclusion alpha-synuclein aggregates with high specificity and sensitivity, showing pathology precedes Lewy body formation in Parkinson's disease.

NanoPlex enables multiplex fluorescence microscopy using nanobodies with erasable signals, achieving up to 21 targets in 3D confocal imaging.

This paper introduces pHluorin-conjugated secondary nanobodies for measuring synaptic vesicle exo- and endocytosis without genetic manipulation.

This study introduces PulsPhos, a method to quantify site-specific phosphorylation lifetimes in living cells and applies it to pharmacological perturbations.

The paper identifies calcium-dependent ubiquitination events in synapses, showing that ubiquitination of CaMKII and AP180 modulates synaptic function through autophosphorylation.

This study shows that erythropoietin (EPO) reduces inhibitory potential in hippocampal interneurons, enhancing pyramidal neuron excitability and potentially improving neuropsychiatric symptoms.

This study shows that IGF1R regulates firing rate homeostasis by modulating mitochondrial calcium uniporter function in neurons.

The paper identifies a novel ECM remodeling mechanism through endocytosis and resurfacing of Tenascin-R near synapses, occurring within 3 days and dependent on activity.

This study shows human brain organoids can form bilateral optic vesicles with cellular diversity and functionality, including light-responsive photoreceptors and neuronal networks.

This study uses super-resolution microscopy to examine the molecular structure of alpha-synuclein inclusions in cell models and human brain tissue, revealing conserved patterns and shapes.

This study shows that continuous myelin synthesis is necessary for maintaining white matter integrity, as preventing new myelin formation in adult mice leads to myelin degradation and neurological disease.