Mitchell G. Thompson

This paper introduces a membrane-free spot-plating protocol for Agrobacterium-mediated transformation of diverse yeasts, improving efficiency and reducing costs.

The paper models variability in Nicotiana benthamiana transient expression and provides guidelines for minimizing it through experimental design and power analysis.

This study shows that binary vector origin of replication family, not copy number, determines Agrobacterium-mediated transformation outcomes across plants and fungi.

This study uses RB-TnSeq to identify genetic basis of dicarboxylic acid metabolism in β-proteobacteria, revealing distinct metabolic pathways and enabling plastic waste upcycling.

This paper introduces a simplified, fast, and efficient method to extract amatoxins from Amanita phalloides without tissue grinding or long incubation.

The paper introduces a family of 43 expression plasmids for precise gene expression in gram-negative bacteria, with constitutive and inducible systems tested in Escherichia coli and Pseudomonas putida.

This paper engineers Pseudomonas putida KT2440 to produce medium- and long-chain free fatty acids and oleochemicals, achieving titers up to 670 mg/L.

The paper identifies an iron-dependent enzyme in plants that completes the lysine catabolism pathway, showing structural and functional conservation across species.

The paper develops a highly sensitive biosensor for caprolactam and valerolactam from Pseudomonas putida, capable of detecting product titers above background at ~6 g/L.

The paper characterizes two glutarate-sensing transcription factors in Pseudomonas putida and develops biosensors to detect glutarate flux for metabolic engineering.

This study identifies and disrupts genes in Pseudomonas putida that cause unwanted metabolism of valerolactam, increasing its production titer.