Advancing Nuclei Isolation from Frozen Human Heart for Single-Nucleus RNA Sequencing Applications
Rocco Caliandro, Ruggero Belluomo, Jermo Hanemaaijer-van der Veer, Roelof‐Jan Oostra, Maurice J.B. van den Hoff, Reinier Abraham Boon, Monika M Gladka
This paper presents a standardized nuclei isolation protocol for frozen human heart tissue and a downstream snRNA-seq analysis pipeline, improving yield and consistency in cardiac single-nucleus transcriptomic studies.
bioRxiv · Mar 21, 2026 · PreprintMolecular Biology