Molecular BiologybioRxivPreprint
Efficient tagging and purification of endogenous proteins for structural studies by single particle cryo-EM
Jianhua Zhao, Suraj Makhija, Bo Huang, Yifan Cheng
The paper describes a method combining CRISPR/Cas and fluorescence cell sorting to efficiently tag and purify endogenous human proteins for structural analysis using cryo-EM.
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