Rapid 40 kb genome construction from 52 parts
John M. Pryor, Vladimir Potapov, Nilisha Pokhrel, Gregory J. S. Lohman
The paper describes a method to rapidly assemble a 40 kb genome from 52 DNA parts using optimized Golden Gate assembly, resulting in infectious phage particles.
bioRxiv · Dec 22, 2020 · PreprintSynthetic Biology