Gregory J. S. Lohman

Single amino acid substitution in DNA Polymerase I dramatically alters infection dynamics of bacteriophage T7

This study shows that a single amino acid substitution in phage T7's DNA polymerase I significantly affects infection dynamics in the T7-E. coli system.

One-pot Golden Gate Assembly of an avian infectious bronchitis virus reverse-genetics system

This paper develops a Golden Gate Assembly-based reverse genetics system for avian infectious bronchitis virus to enable rapid generation of mutants and gene replacements.

Rapid 40 kb genome construction from 52 parts

The paper describes a method to rapidly assemble a 40 kb genome from 52 DNA parts using optimized Golden Gate assembly, resulting in infectious phage particles.

Optimization of Golden Gate assembly through application of ligation sequence-dependent fidelity and bias profiling

The paper develops a method to optimize Golden Gate assembly by profiling ligation fidelity and bias, enabling efficient assembly of up to 24 fragments in a single reaction.