Author
New England Biolabs (United States)ORCID 0000-0001-7000-9383Unclaimed ORCID profile · follow to get notified of new preprints
Mutual co-authors in cluster
3 papers
Recent·Most cited
Katharina Bilotti, Sarah Keep, Andrew P. Sikkema…·Erica Bickerton
This paper develops a Golden Gate Assembly-based reverse genetics system for avian infectious bronchitis virus to enable rapid generation of mutants and gene replacements.
John M. Pryor, Vladimir Potapov, Nilisha Pokhrel, Gregory J. S. Lohman
The paper describes a method to rapidly assemble a 40 kb genome from 52 DNA parts using optimized Golden Gate assembly, resulting in infectious phage particles.
Vladimir Potapov, Jennifer L. Ong, Rebecca B. Kucera…·Gregory J. S. Lohman
The paper develops a method to optimize Golden Gate assembly by profiling ligation fidelity and bias, enabling efficient assembly of up to 24 fragments in a single reaction.