Danielle Biscaro Pedrolli

Rational design of intrinsic transcription terminators with programmable strength

The paper designs intrinsic transcription terminators with programmable strength using a sequence-to-function model and provides web tools for prediction and design.

LeDNA: a cut-and-build toolkit to democratize education on CRISPR gene editing technology

LeDNA provides an open-source, affordable educational toolkit for teaching CRISPR gene editing and genetics across educational levels.

Signal-amplification for cell-free biosensors, an analog-to-digital converter

The paper introduces a signal-amplification circuit that reduces leakage and improves sensitivity in toehold switches for RNA detection.

Preventing production escape during scale-up using an engineered glucose-inducible genetic circuit

The paper engineers a glucose-inducible genetic circuit in Bacillus subtilis to maintain production capacity during scale-up by switching metabolic states, improving industrial production efficiency.

Characterization of five purine riboswitches in cellular and cell-free expression systems

This study characterizes five purine riboswitches in both in vitro and in vivo systems, identifying transcription pause sites and correlating readthrough with gene expression.

Targeting riboswitches with synthetic small RNAs for metabolic engineering

This paper designs synthetic small RNAs to target bacterial riboswitches, enhancing gene expression up to 103-fold and improving riboflavin production in engineered strains.