Synthetic BiologybioRxivPreprint
BatCRISPRi: Bacillus titratable CRISPRi for dynamic control in Bacillus subtilis
Andrés F. Flórez, Sebastian M. Castillo-Hair, Luis Gutiérrez-López…·Ethan Clark Garner
This paper introduces BatCRISPRi, a CRISPRi system in Bacillus subtilis that uses a rapamycin-dependent degron to eliminate leakiness and enable reversible gene knockdown.