Johan Paulsson

The paper uses a high-throughput platform to measure single-cell dynamics of gene-regulatory circuits and maps their design space by testing robustness and identifying key interactions.

The paper investigates bacterial warfare by analyzing toxin production in Escherichia coli, finding that localized toxin bursts are more effective than uniform production in structured populations.

The paper shows how inherited flagellar structures coordinate swimming onset in E. coli cells by integrating transcriptional noise and sharpening it into a heritable decision.

This study measures within-cell plasmid fitness and shows that incompatible plasmids co-occur longer due to methylation-based eclipsing, with less transcriptionally active plasmids having a selective advantage.

The paper uses multi-generational imaging to study essential genes in E. coli, revealing new mechanisms behind growth laws and identifying unknown gene functions.

This paper introduces BatCRISPRi, a CRISPRi system in Bacillus subtilis that uses a rapamycin-dependent degron to eliminate leakiness and enable reversible gene knockdown.

The paper constructs RNA-based synthetic oscillators using deactivated CRISPR-Cas systems in bacterial cells, finding that dCas12a oscillators perform better than dCas9 oscillators.

This study uses a synthetic gene oscillator to analyze bacterial growth dynamics in the gut under healthy and inflammatory conditions, revealing variability in single-cell behavior.

The paper quantifies very low-abundant proteins in bacteria using HaloTag and epifluorescence microscopy, achieving ≥80% detection efficiency.