Toma Kashima

Discovery and structural analysis of glycoside hydrolase family 176 α-1,2 glucosidase from Arthrobacter humicola A8F5

This paper discovers and structurally analyzes a novel -1,2-glucosidase from Arthrobacter humicola A8F5, revealing its unique mechanism and structural features for cleaving rare -1,2-glucosidic bonds.

Structural mechanism defining product specificity in glycoside hydrolase family 66 cycloisomaltotetraose glucanotransferase

This study identifies a molecular mechanism in CI4Tase that enforces strict DP4 specificity for cycloisomaltotetraose synthesis through structural and mutational analysis.

Reprogramming protein interfaces between adenylation and carrier protein domains in nonribosomal peptide synthetases

This study reprograms protein interfaces between adenylation and carrier protein domains in NRPSs to enable non-cognate pairings and enhance natural product production.

Crystal structure of plant γ-glutamyl peptidase 1 with dual roles in sulfur metabolism and implications for oxidative stress regulation

This study determines the crystal structures of Arabidopsis thaliana GGP1, revealing its dual role in sulfur metabolism and its potential involvement in oxidative stress regulation.

Neutron crystallography of the covalent intermediate of β-glucosidase reveals remodeling of the catalytic center

This paper uses neutron crystallography to determine the structure of β-glucosidase Td2F2 in different ligand states, revealing hydrogen bonding networks and structural changes in the catalytic center during covalent intermediate formation.

Discovery of a wide variety of α-1,6-cyclized β-1,2-glucan synthases: a new entrance for host-microbe interactions.

The paper identifies and characterizes α-1,6-cyclized β-1,2-glucan synthases, revealing their role in plant-pathogen interactions and potential applications in agriculture.

Structural basis for the strict substrate specificity of beta-D-galactofuranosidase from Streptomyces sp. JHA19

The paper determines the crystal structure of a Galf-specific beta-D-galactofuranosidase from Streptomyces sp. JHA19, revealing the structural basis for its strict substrate specificity.