Laercio R. Porto Neto

Optimizing Genome Editing in Bovine Cells: A Comparative Study of Cas9 Variants and CRISPR Delivery Methods

This study compares Cas9 variants and delivery methods for CRISPR gene editing in bovine cells, finding that commercial Alt-R HiFi SpCas9 and Sniper2L achieve high deletion efficiency, while Neon electroporation is better for knock-ins.

Correlation scan: identifying genomic regions that affect genetic correlations applied to fertility traits

This paper introduces a framework to identify genomic regions affecting genetic correlations between male and female fertility traits in cattle using high-density SNP data.

Genome-wide co-expression distributions as a metric to prioritize genes of functional importance

The paper introduces a method to analyze genome-wide co-expression distributions to identify genes with functional importance by comparing distribution shapes across species.

Association analysis of loci implied in “buffering” epistasis

This paper develops a statistical model to detect buffering loci in genome-wide association studies and identifies loci with significant epistatic effects but no additive effects in bovine data.

Systems biology reveals NR2F6 and TGFB1 as key regulators of feed efficiency in beef cattle

The paper identifies NR2F6 and TGFB1 as key regulators of feed efficiency in beef cattle using systems biology approaches and validates their roles in hepatic inflammation and muscle development.

Mammalian genomic regulatory regions predicted by utilizing human genomics, transcriptomics and epigenetics data

The paper develops a computational method to predict regulatory regions in mammals using human genomics data, and applies it to cattle to prioritize variants and identify genome editing targets.