Xiaofeng Du

This study compares Cas9 variants and delivery methods for CRISPR gene editing in bovine cells, finding that commercial Alt-R HiFi SpCas9 and Sniper2L achieve high deletion efficiency, while Neon electroporation is better for knock-ins.

This study applies CRISPR interference to repress SmfgfrA in Schistosoma mansoni, showing reduced transcription, protein expression, and immune response, with durable effects on parasite development and host granuloma formation.

This paper uses CRISPR/Cas9 to edit the acetylcholinesterase gene in Schistosoma mansoni eggs, resulting in reduced AChE activity and decreased granuloma size in mice.