Charles M. Boone

The paper introduces MOSAIC, a database and web resource for chemical-genetic interaction data, enabling mode-of-action prediction and discovery of compound effects in yeast.

This study develops a yeast-based chemical-genetic screening system to annotate compound functionality across biological processes and identifies compounds with dual modes of action.

The paper evaluates CRISPR/Cas9 knockout screens in human cell lines and designs an optimized gRNA library to improve gene knockout efficiency and accuracy.

The paper measures pleiotropy in yeast genes using genetic interaction networks, finding high-pleiotropy genes have broader functional influence and distinct characteristics compared to low-pleiotropy genes.