Molecular BiologyPLoS ONEPeer-reviewed
Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency
Ignacio García‐Tuñón, Verónica Alonso-Pérez, Elena Vuelta…·Manuel Sánchez‐Martín
This study shows that targeting splice donor sites with sgRNAs improves CRISPR/Cas9 knockout efficiency by increasing frameshift mutations and disrupting pre-mRNA splicing.
26 citations