Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency
Ignacio García‐Tuñón, Verónica Alonso-Pérez, Elena Vuelta, Sandra Pérez‐Ramos, María Herrero, Lucía Méndez, Jesús María Hernández-Sánchez, Marta Martín‐Izquierdo, Raquel Saldaña, Julián Sevilla, Fermin M. Sanchez-Guijo, Jesús María Hernández‐Rivas, Manuel Sánchez‐Martín
This study shows that targeting splice donor sites with sgRNAs improves CRISPR/Cas9 knockout efficiency by increasing frameshift mutations and disrupting pre-mRNA splicing.
PLoS ONE · May 9, 2019Peer-reviewedMolecular Biology