A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes
Yury S. Bykov, Solène Zuttion, Dunya Edilbi, Marina Polozova, Johanna Arnold, Sergey Malitsky, Maxim Itkin, Bruno Senger, Ofir Klein, Yeynit Asraf, Hadar Meyer, Hubert D Becker, Róża Kucharczyk, Maya Schuldiner
The paper uses a bi-genomic split-GFP assay to identify mitochondrial matrix and inner membrane proteins, revealing 50 previously unvisualized proteins and characterizing dually localized proteins.
bioRxiv · Apr 17, 2024Cell Biology