Joseph M. Dobbs

This paper introduces a modular cryogenic light microscope for single-molecule localization microscopy, improving stability and reducing ice contamination in cryo-CLEM.

This paper demonstrates that cryo-FIB lamellae allow higher excitation laser intensities in SR-cryo-CLEM, improving localization precision and imaging speed.

The paper optimizes data quality and completeness in cryo-ET experiments by analyzing processing parameters to improve annotation and structural characterization of macromolecular complexes.

The paper uses cryo-electron tomography to visualize bacterial translation processes in Mycoplasma pneumoniae, revealing structural coordination between transcription, translation, and membrane attachment.

This study investigates how cooling temperature, immersion medium, and EM grid material affect devitrification in cryo-SMLM, showing that metallic supports in cold nitrogen tolerate higher laser intensities.