Jonas Ries

Super-resolution mapping of platelet α-granules uncovers non-polarized secretion and differential retention of von Willebrand Factor and thrombospondin-1

This study uses super-resolution microscopy and computational analysis to show that platelet α-granules release cargo without polarity, with distinct retention mechanisms for TSP1 and VWF.

Single-molecule reaction chambers reveal intramolecular protein kinase activation

The paper demonstrates that protein kinases can undergo intramolecular autophosphorylation using single-molecule reaction chambers, challenging the conventional view of intermolecular activation.

Microscope control with a natural language agent

The paper introduces MicroClaw, an AI agent that automates complex microscopy workflows across platforms without requiring expert knowledge.

Multi-channel high-density single-molecule localization

This paper introduces DECODE-Plex, a deep learning framework for multi-channel single-molecule localization microscopy, improving imaging speed and quality in dense, overlapping data.

A stable cryogenic fluorescence microscope for correlative super-resolution light and electron microscopy

This paper introduces a modular cryogenic light microscope for single-molecule localization microscopy, improving stability and reducing ice contamination in cryo-CLEM.

Cryo-FIB lamellae enable ultra-high excitation laser intensities for cryogenic super-resolution fluorescence imaging

This paper demonstrates that cryo-FIB lamellae allow higher excitation laser intensities in SR-cryo-CLEM, improving localization precision and imaging speed.

Continuum architecture dynamics of vesicle tethering in exocytosis

The paper uses multimodal imaging to study the dynamic structure of the exocyst complex during exocytosis, revealing its conformational changes and tethering mechanism.

Evaluating MINFLUX experimental performance in silico

The paper evaluates MINFLUX performance using a simulator to identify limitations from fluorophore dynamics, background, estimators, and misalignment.

Multi-step implementation of meiotic crossover patterning

The paper investigates the timing and mechanism of crossover designation in C. elegans using advanced imaging techniques, revealing a multi-step, dynamic regulation process.

Multiscale imaging of cytokinesis

This study develops a multimodal imaging framework to analyze nanoscale reorganization of cytokinetic proteins during cell division, revealing a new substep and creating detailed molecular maps.

Effects of base temperature, immersion medium, and EM grid material on devitrification thresholds in cryogenic optical super-resolution microscopy

This study investigates how cooling temperature, immersion medium, and EM grid material affect devitrification in cryo-SMLM, showing that metallic supports in cold nitrogen tolerate higher laser intensities.

Chlamydial membrane vesicles deliver the chlamydial porin OmpA to mitochondria to inhibit apoptosis

This study shows that Chlamydia trachomatis uses membrane vesicles to deliver OmpA to mitochondria, inhibiting apoptosis and promoting bacterial survival in human cells.

Simulating structurally variable Nuclear Pore Complexes for Microscopy

The paper introduces a simulation pipeline to generate structurally variable synthetic NPCs for benchmarking image analysis methods and understanding NPC variability in SMLM data.

Global fitting for high-accuracy multi-channel single-molecule localization

This paper introduces globLoc, a GPU-based global fitting algorithm for multi-channel single-molecule localization, improving 3D precision and color assignment in SMLM.

EMU: reconfigurable graphical user interfaces for Micro-Manager

EMU is a Micro-Manager plugin that simplifies creating flexible, reconfigurable user interfaces for microscopes by allowing easy mapping of device properties to interface functions.

Organotypic slice culture model demonstrates interneuronal spreading of alpha-synuclein aggregates

The paper demonstrates that alpha-synuclein aggregates spread interneuronally in an organotypic hippocampal slice model, independent of serine 129 phosphorylation.

Three dimensional particle averaging for structural imaging of macromolecular complexes by localization microscopy

This paper introduces a 3D particle averaging method for localization microscopy that improves signal-to-noise ratio and resolution without requiring a structural template.

Topological data analysis quantifies biological nano-structure from single molecule localization microscopy

The paper introduces a topological data analysis method for analyzing single molecule localization microscopy data, improving segmentation and quantifying nano-structure.

ChromoTrace: Reconstruction of 3D Chromosome Configurations by Super-Resolution Microscopy

The paper develops ChromoTrace, a method to reconstruct 3D chromosome configurations from super-resolution microscopy images with high accuracy and sensitivity.

A real-time compression library for microscopy images

The paper introduces a real-time compression library for microscopy images, offering fast lossless and lossy compression with high compression ratios.