Advanced 3D spheroid-based skin models and deep-learning based image analysis enable in-depth investigation of keratinocyte differentiation and barrier function
Tiziana Cesetti, Claudia Buerger, Nathalie Couturier, Elina Nürnberg, Roman Bruch, Victoria Lang, Mathias Hafner, Markus Reischl, Torsten Fauth, Rüdiger Rudolf
The paper introduces 3D spheroid-based skin models and a deep-learning pipeline to study keratinocyte differentiation and barrier function, identifying NHK-E6/E7 as the best alternative to primary keratinocytes.
bioRxiv · Jan 23, 2026 · PreprintBioengineering