DNA-terminus-dependent transcription by T7 RNA polymerase and its C-helix mutants
Bingbing Yu, Yifan Chen, Yan Yan, Xueling Lu, Bin Zhu
This study identifies DNA-terminus-initiated transcription by T7 RNA polymerase as the main source of dsRNA byproducts and shows that a C-helix mutant reduces dsRNA production and improves mRNA quality.
Nucleic Acids Research · Jul 9, 2024Peer-reviewedMolecular Biology