Efficient Formation of Single-copy Human Artificial Chromosomes
Craig W. Gambogi, Gabriel J. Birchak, Elie Mer, David Brown, George Yankson, Kathryn Kixmoeller, Janardan N. Gavade, Josh L. Espinoza, Prakriti Kashyap, Christopher L. Dupont, Glennis A. Logsdon, Patrick Heun, John I. Glass, Ben E. Black
This paper describes a method to efficiently form single-copy human artificial chromosomes by using a large construct and yeast spheroplast fusion to avoid multimerization.
Science · Mar 21, 2024Peer-reviewedMolecular Biology