John I. Glass

Full-length transcriptomics and proteomics reveal how genome minimization reshapes gene expression in synthetic bacteria

The paper characterizes RNA and protein expression in synthetic bacteria Syn1.0 and Syn3A, showing genome minimization alters gene expression and resource allocation.

Selection-free whole genome transplantation revives dead microbes

The paper demonstrates a method to revive dead microbes by transplanting synthetic genomes, eliminating the need for antibiotic resistance markers in the process.

Bringing the Genetically Minimal Cell to Life on a Computer in 4D

A hybrid 4D whole-cell simulation of the minimal bacterium JCVI-syn3A integrates spatial reaction-diffusion, metabolism, polymer chromosome dynamics, and division across a complete 105-minute cell cycle.

Protein-only centromeric chromatin assembly streamlines human artificial chromosome formation

This paper describes the creation of a 2 Mb human artificial chromosome (HAC) using a protein-only centromere seeding approach that bypasses genetic engineering of target cells.

Rapid assembly of functional modules for generating human artificial chromosome constructs compatible with epigenetic centromere seeding

This paper describes a method to rapidly assemble functional modules for human artificial chromosomes (HACs) that can be seeded with epigenetic centromere components.

Unraveling the Transcriptional Landscape within a Minimized Bacterium via Comparative Analysis

This study compares transcriptomes of two minimized bacteria to understand gene expression patterns and highlights limitations of bioinformatics alone.

Robust and highly efficient transformation method for a minimal Mycoplasma cell

This paper introduces a highly efficient transformation method for the minimal Mycoplasma cell JCVI-syn3B, achieving transformation efficiencies up to 4.4 x 10^-2 with minimal DNA and cell amounts.

PEG Adjustment Enables Genome Transplantation Using Mycoplasma Mycoides Recipient

This study shows that increasing PEG concentration allows successful genome transplantation using Mycoplasma mycoides as recipient cells, expanding the potential for synthetic biology applications.

Efficient Formation of Single-copy Human Artificial Chromosomes

This paper describes a method to efficiently form single-copy human artificial chromosomes by using a large construct and yeast spheroplast fusion to avoid multimerization.

High content screening and computational prediction reveal viral genes that suppress innate immune response

The paper develops a high-content assay to identify viral genes that suppress innate immunity and validates known and novel immune suppressors from various viral families.

Genetic requirements for cell division in a genomically minimal cell

Seven genes, including ftsZ, sepF and five of unknown function, are jointly required to restore near-normal morphology and division in the minimal cell JCVI-syn3.0.

High-content screening of coronavirus genes for innate immune suppression revealsenhanced potency of SARS-CoV-2 proteins

The paper screens coronavirus proteins for innate immune suppression, finding SARS-CoV-2 proteins are more potent immune suppressors than other coronaviruses.