Prakriti Kashyap

This paper describes the creation of a 2 Mb human artificial chromosome (HAC) using a protein-only centromere seeding approach that bypasses genetic engineering of target cells.

This paper describes a method to rapidly assemble functional modules for human artificial chromosomes (HACs) that can be seeded with epigenetic centromere components.

This paper describes a method to efficiently form single-copy human artificial chromosomes by using a large construct and yeast spheroplast fusion to avoid multimerization.