Maria Spiliopoulou

Best practices for cryo-trapping time-resolvedcrystallography with the Spitrobot crystal plunger

This paper describes a protocol for cryo-trapping time-resolved crystallography using automated crystal plungers to study enzyme reaction pathways at millisecond resolution.

Structural analysis of Helicobacter pylori glutamate racemase in a monoclinic crystal form

The paper presents a high-resolution crystal structure of Helicobacter pylori glutamate racemase in monoclinic symmetry, highlighting differences in crystal packing due to unit-cell parameter variations.

Multi-state Ensemble Refinement for Occupancy Statistics (MEROS) in Time-Resolved X-ray Crystallography

MEROS assesses occupancy-ADP statistics for overlapping states in time-resolved crystallography using Monte Carlo sampling and ensemble refinement.

Tracking ligand-binding-induced structural populations in T4 lysozyme by time-resolved serial crystallography

This study uses time-resolved serial crystallography to track ligand binding and conformational changes in T4 lysozyme, revealing a diffusion-limited process leading to a dominant conformational state.

SPITROBOT-2 advances time-resolved cryo-trapping crystallography to under 25 ms

SPITROBOT-2 improves cryo-trapping crystallography with a 23 ms delay, reducing sample preparation time to three minutes per sample.