P. Mehrabi

This paper describes a protocol for cryo-trapping time-resolved crystallography using automated crystal plungers to study enzyme reaction pathways at millisecond resolution.

SPITROBOT-2 improves cryo-trapping crystallography with a 23 ms delay, reducing sample preparation time to three minutes per sample.

This study shows that subtle mutations at the dimer interface of FAcD affect enzyme activity by disrupting hydrogen-bonding networks and altering dimer stability.

This paper introduces a method for rapid protein structure determination using serial electron diffraction in a scanning transmission electron microscope, achieving high-resolution results with minimal sample usage.