Isolation of Biologically Active Extracellular Vesicles-Associated AAVs for Gene Delivery to the Brain by Size Exclusion Chromatography
Carina Henriques, Miguel Monteiro Lopes, Patrícia Maia Correia de Albuquerque, David Rufino‐Ramos, Laetitia S. Gaspar, Diana Duarte Lobo, Kevin Leandro, Ana Carolina da Silva, Rafael Baganha, Sónia Duarte, Casey A. Maguire, Magda M. Santana, Luís Pereira de Almeida, Rui Jorge Nobre
This paper optimizes size exclusion chromatography (SEC) for isolating biologically active EV-AAVs for non-invasive brain gene delivery, showing higher recovery and transgene expression efficiency compared to differential ultracentrifugation.
bioRxiv · May 30, 2023 · PreprintMolecular Biology