Cas9 RNP Physiochemical Analysis for Enhanced CRISPR-AuNP Assembly and Function
Daniel D. Lane, Karthikeya S.V. Gottimukkala, Rachel A. Cunningham, Youngseo Jwa, Molly E. Cassidy, Jack M.P. Castelli, Jennifer E. Adair
This paper improves CRISPR-AuNP delivery by optimizing Cas9 RNP loading and nanoparticle surface chemistry, achieving higher RNP loading and uptake in HSPCs but with limited endosomal escape.
bioRxiv · Apr 2, 2024 · PreprintBioengineering