BioengineeringbioRxivPreprint
Cas9 RNP Physiochemical Analysis for Enhanced CRISPR-AuNP Assembly and Function
Daniel D. Lane, Karthikeya S.V. Gottimukkala, Rachel A. Cunningham…·Jennifer E. Adair
This paper improves CRISPR-AuNP delivery by optimizing Cas9 RNP loading and nanoparticle surface chemistry, achieving higher RNP loading and uptake in HSPCs but with limited endosomal escape.
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