Author

Darren E. Lee

Western Washington UniversityORCID 0000-0002-5053-863XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Organism:bacteria (other)· 1 Approach:experimental assay· 1 Research Focus:protein folding & dynamics· 1 Assay:protein structure· 1

Frequent Co-Authors

Caroline CeravoloiD· 1 shared Jadon M. BlountiD· 1 shared James McCartyiD· 1 shared Jeanine F. AmacheriD· 1 shared John M. AntosiD· 1 shared Justin W. Ibershof· 1 shared Kate M. Brown· 1 shared Kayla A. Croney· 1 shared

Activity by Year

2025-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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1 shared paperBiochemistry

Caroline CeravoloORCID

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1 paper

Recent·Most cited

Biochemical characterization of Bacillus anthracis sortase B: Use in sortase mediated ligation and substrate recognition dependent on residues beyond the canonical pentapeptide binding motif for sortase enzymes

Sophie N. Jackson, Darren E. Lee, Jadon M. Blount, Kayla A. Croney, Justin W. Ibershof, Caroline Ceravolo, Kate M. Brown, Noah J. Goodwin-Rice, Kyle M. Whitham, James McCarty, John M. Antos, Jeanine F. Amacher

This study characterizes Bacillus anthracis SrtB, showing that residues outside the canonical pentapeptide motif are critical for activity and that engineered variants can improve ligation efficiency.

Journal of Biological Chemistry · Mar 4, 2025Peer-reviewedBiochemistry