Low copy CRISPR-Cas13d mitigates collateral RNA cleavage
Sydney K. Hart, Simon Müller, Hans‐Hermann Wessels, Alejandro Méndez‐Mancilla, Gediminas Drabavicius, Olivia Choi, Neville E. Sanjana
The paper evaluates collateral RNA cleavage in CRISPR-Cas13d systems and finds that low-copy expression reduces off-target effects while maintaining on-target knockdown.
Nature Biotechnology · Feb 11, 2025Peer-reviewedGenomics