Simon Müller

Transcriptome-wide profiling of alternative splicing regulators with CRISPore-seq

CRISPore-seq combines CRISPR perturbations with long-read transcriptomics to detect alternative splicing changes, revealing that exon skipping is common and linked to RNA-binding protein perturbations.

Low copy CRISPR-Cas13d mitigates collateral RNA cleavage

The paper evaluates collateral RNA cleavage in CRISPR-Cas13d systems and finds that low-copy expression reduces off-target effects while maintaining on-target knockdown.

RNA-seq derived sequence variations are excellent features for cell line identification

This study shows RNA-seq-derived sequence variations can reliably identify cell lines and detect cross-contamination using machine learning.

RAVER1 interconnects lethal EMT and miR/RISC activity by the control of alternative splicing

This paper investigates RAVER1's role in controlling alternative splicing and its impact on miR/RISC activity and EMT in cancer cells.

Combinatorial recognition of clustered RNA elementsby a multidomain RNA-binding protein, IMP3

The paper identifies the combinatorial RNA recognition code of IMP3 using an integrative approach combining SELEX-seq, iCLIP, and structural analysis.