J Hruby

This paper introduces a method using ultrasonic excitation in plunge freezing tweezers to reduce preferred particle orientation in cryo-EM, achieving a complete reconstruction of hemagglutinin at 2.5 Å resolution.

This paper demonstrates a method to rehydrate proteins after soft-landing for cryo-EM by flash melting, enabling native configurations and improving sample preparation.

This paper introduces a method to achieve microsecond time resolution in cryo-EM using jet vitrification, enabling observation of protein dynamics with near-atomic resolution.

This paper introduces a method to reduce preferred orientation in cryo-EM samples using laser flash melting and revitrification, improving protein structure determination.