A protocol for locating and counting transgenic sequences from laboratory animals using a map-then-capture (MapCap) sequencing workflow: procedure and application of results.
Sidney S. Dicke, Clifford Dustin Rubinstein, James M. Speers, Mark E. Berres, Derek M. Pavelec, Kathryn L. Schueler, Donnie S. Stapleton, Shane P. Simonett, Mark P. Keller, Alan Attie, Martin T. Zanni
The paper presents a MapCap sequencing workflow to locate and count transgenic sequences in mice, identifying insertion sites and characterizing genetic variations in a diabetes model.
bioRxiv · Jan 13, 2022 · PreprintGenetics