BiochemistrybioRxivPreprint
From Plasmid to Pure Protein: Production and Characterization of SARS-CoV-2 PLpro
Anna De Falco, Rebecca Greene-Cramer, Ben A. Shurina…·Gaetano Thomas Montelione
This paper provides protocols for producing and characterizing native SARS-CoV-2 PLpro in E. coli with high yields and supports inhibitor studies using NMR and fluorescence assays.
DOI 10.1101/2025.03.09.642282