Björn O. Forsberg

This paper introduces a normalized local filter to estimate compositional heterogeneity in cryo-EM maps and demonstrates its utility for interpretation and signal subtraction.

The paper resolves the structure of the fungal E3-binding protein (E3BP) in complex with the PDC core and shows its orthology to mammalian E3BP, bridging evolutionary distance and suggesting E3-specificity discriminants.

The paper uses cryo-EM to study the structure of the fungal pyruvate dehydrogenase complex, revealing two PX oligomer arrangements that explain stoichiometry variation and suggest therapeutic targets.