The paper shows clade II N2O-reducers outcompete clade I under low N2O availability, and coexist under high N2O, with cobalamin interactions influencing community composition.

ISME Communications · Jan 1, 2025Peer-reviewedSystems Biology

Influence of DNA extraction methods on microbiome and resistome analysis in activated sludge

David Calderón-Franco, Dana Kok, Renzo Dukker, Ben A. Abbas, Joana Abreu-Silva, Jaqueline Abel da Rocha, Marco Antonio Lopez Marin, Rebeca Pallarés-Vega, Stanislav Gajdoš, Mythili Ananth, Seeram Apoorva, Ivan Karpíšek, Milada Šolcová, Lucía Hernández Leal, Jan Bartáček, , David Gregory Weissbrodt

This study evaluates how DNA extraction methods affect microbiome and resistome analysis in activated sludge, finding significant variability in results depending on the technique used.

bioRxiv · Jun 26, 2023 · PreprintMolecular Biology

A novel method to isolate free-floating extracellular DNA from wastewater for quantitation and metagenomic profiling of mobile genetic elements and antibiotic resistance genes

David Calderón-Franco, Mark C.M. van Loosdrecht, Thomas Abeel, David Gregory Weissbrodt

Water Research · Nov 2, 2020Peer-reviewedMolecular Biology

Biomass segregation between biofilm and flocs improves the control of nitrite-oxidizing bacteria in mainstream partial nitritation and anammox processes

Michele Laureni, David Gregory Weissbrodt, Kris Villez, Orlane Robin, Nadieh de Jonge, Alex F. Rosenthal, George Wells, Jeppe Lund Nielsen, Eberhard Morgenroth, Adriano Joss

The paper investigates how biomass segregation between biofilm and flocs affects nitrite-oxidizing bacteria control in hybrid PN/A systems, showing that low DO promotes NOB washout from flocs.

Water Research · Jan 7, 2019Peer-reviewedSystems Biology