Mark C.M. van Loosdrecht

The paper shows glycogen-accumulating organisms use the ethylmalonyl-CoA pathway for CO2 fixation to achieve metabolic parity with polyphosphate-accumulating organisms, leading to community shifts through stoichiometric advantages.

SugarBase identifies microbial nucleotide sugar profiles, revealing species-specific and previously unannotated sugars, and expands antimicrobial targets.

The paper develops a wastewater metaproteomics approach to identify microbial and human protein biomarkers from wastewater samples over three months.

Meta-PepView evaluates and visualizes metaproteomics data through a web-based platform, offering modular design and open-source access.

The paper develops a metabolomics workflow to measure temporal variations in nucleotide pools in EBPR biofilms, revealing dynamic changes in energy charge during metabolic processes.

This study uses long-term multi-meta-omics to identify the ecophysiological controls of seasonal N2O emissions in wastewater treatment systems.

This study shows that dual substrate uptake by 'Candidatus Accumulibacter' improves metabolic efficiency in EBPR by reducing ATP losses through optimized storage polymer use.

The paper combines microbial enrichment with metagenomics and metaproteomics to identify novel glycoside hydrolases from complex environments.

The paper shows clade II N2O-reducers outcompete clade I under low N2O availability, and coexist under high N2O, with cobalamin interactions influencing community composition.

This study shows two Aeromonas strains can degrade and use chitin as a carbon and nitrogen source, revealing their metabolic potential in nutrient-poor environments.

The paper uses metaproteomic approaches to analyze the extracellular proteome of a granular Candidatus Accumulibacter enrichment, identifying proteins involved in biofilm formation.

This study uses FT-IR micro-spectroscopy to analyze the spatial distribution of extracellular matrix components in biofilms, identifying polysaccharide and protein gradients and their correlations.

This study shows that mild aeration in sand filters promotes biological iron removal, achieving faster oxidation rates and higher water yields compared to intensive aeration.

This study identifies Fe3+ flocs as the main cause of nitrification delay in groundwater filters by inhibiting NH4+ oxidation, showing the inhibition is reversible through backwashing.

NovoLign provides a de novo metaproteomics pipeline for rapid taxonomic profiling and reference database creation, offering an alternative to database-based spectral annotation.

The paper compares metaproteomic and genomic approaches to study the aerobic granular sludge microbiome, highlighting differences in insights and the importance of reference databases.

This paper introduces a mass binning glycoproteomics approach to explore prokaryotic protein glycosylation, revealing unique surface layer modulation in an anammox bacterium.

This paper introduces a de novo metaproteomics pipeline and validation strategy to enable quantitative profiling of microbial communities without relying on sequence databases.

The paper models how different metabolic strategies in microbial communities evolve under dynamic oxygen availability, showing how storage metabolism affects competitive advantage.