Native mass spectrometry of membrane protein lipid and ligand complexes using de novo designed WRAP domains
Surabhi Kokane, Ljubica Mihaljević, Barbara Abreu, Stijn de Schepper, Alexander Stevens, Erik G. Marklund, David A. Baker, Phillip J. Stansfeld, Michael Landreh
The paper demonstrates that WRAP domains designed with deep learning can preserve weak interactions of membrane protein complexes in native mass spectrometry, enabling characterization of lipid and ligand interactions without detergent micelles.
bioRxiv · Jul 25, 2026 · PreprintBiophysics