Ljubica Mihaljević

Native mass spectrometry of membrane protein lipid and ligand complexes using de novo designed WRAP domains

The paper demonstrates that WRAP domains designed with deep learning can preserve weak interactions of membrane protein complexes in native mass spectrometry, enabling characterization of lipid and ligand interactions without detergent micelles.

Topological reprogramming transforms an integral membrane oligosaccharyltransferase into a water-soluble glycosylation catalyst

The paper describes methods to make a membrane-bound glycosyltransferase water-soluble for easier study and application in synthetic glycobiology.

Bottom-up design of calcium channels from defined selectivity filter geometry

This paper designs Ca2+ channels with specific selectivity filter geometries using a bottom-up approach, showing higher Ca2+ conductance and structural accuracy.

De novo design of miniprotein agonists and antagonists targeting G protein-coupled receptors

This paper presents computational methods for designing GPCR-binding miniproteins and validates them through high-throughput screening, producing agonists and antagonists for multiple GPCRs.

Solubilization of Membrane Proteins using designed protein WRAPS

This paper introduces WRAPs, designed proteins that solubilize membrane proteins without detergents, enabling structural studies and vaccine development.

KAT6A deficiency impairs cognitive functions through suppressing RSPO2/Wnt signaling in hippocampal CA3

This study identifies KAT6A's role in regulating synaptic plasticity and memory through RSPO2/Wnt signaling in hippocampal CA3, linking KAT6A deficiency to cognitive impairments.